Electrochemical Analysis of Proteins and Cells by Genxi Li & Peng Miao

Author:Genxi Li & Peng Miao
Language: eng
Format: epub
Publisher: Springer Berlin Heidelberg, Berlin, Heidelberg

Graphene is a newly developed advanced two-dimensional nanomaterial, discovered in the year of 2004 [103]. Graphene has many unique properties including fast electron transportation, high thermal conductivity, excellent biocompatibility, which promise its great application potential in electrochemical analysis of proteins. Zhao et al. [104] have reported a simple and smart electrochemical biosensing platform based on PGE modified with graphene quantum dots with the help of some specific ssDNA added in the test solution. Although the good conductivity of graphene material may result in large electrochemical response of the modified electrode, the single-strand DNA will be strongly bound to graphene material, inhibiting the electron transportation between [Fe(CN)6]3−/4− and the substrate electrode. Nevertheless, when target protein also exists in the test solution, the ssDNA added in the test solution, which is designed as the aptamer of the target protein, will bind with its target, instead of binding with the graphene quantum dots modified on the PGE surface; thus, the peak current of [Fe(CN)6]3−/4− is increased with the increased concentration of the target protein (Fig. 3.14).

Fig. 3.14Differential pulse voltammograms of [Fe(CN)6]3−/4− obtained at a graphene quantum dots–modified electrode for the cases that ssDNA-1 has been incubated by thrombin with different concentrations. Insets show the plots of the peak current against the concentration of ssDNA-2 or thrombin. Reprinted from Ref. [104], Copyright 2011, with permission from Elsevier

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